Professor of Biology
Office: BI232
Phone: (518) 442-4331
Email: [email protected]
Fax: (518) 442-4767
Research Interests:
Biochemistry of
DNA Repair Enzymes
DNA Repair in
Bacteria
Structure and
Function of DNA Lyases [Click here to view
endonuclease III bound to DNA]
Structure and
function of DNA Nucleases [Click here to
view exonuclease III bound to DNA]
Structure and
Function of DNA Glycosylases
Genetic Analysis
of DNA Repair Pathways
Recent Publications:
Kuo,C-H., Thayer,
M.M., Mol, C.D., Cunningham, R.P., and Tainer, J.A. (1994) Structure
and function of the DNA repair enzyme exonuclease III from E.
coli. Annals of the New York Academy of Science 726, 223-235.
O' Donnell, R.E.,
Boorstein, R.J., Cunningham,R.P., and Teebor, G.W. (1994) Effect
of pH and temperature on the stability of UV-induced repairable
pyrimidine hydrates in DNA. Biochemistry 33,9875-9880.
Cunningham, R.P.,
M.M. Thayer and Tainer, J.A. (1994) Structure of the DNA repair
enzyme endonuclease III. Nucleic Acids and Molecular Biology 8,
227-235.
Hatahet, Z., Kow,
Y.W., Purmal, A.A., Cunningham, R.P., and Wallace, S.S. (1994)
New substrates for old enzymes: 5-hydroxy-2' -deoxycytidine and
5-hydroxy-2' - deoxyuridine are substrates for Escherichia coli
endonuclease III and formamidopyrimidine DNA N-glycosylase while
5-hydroxy-2, -deoxyuridine is a substrate for uracil DNA N-glycosylase.
J.Biol.Chem. 269,18814-18820.
O' Handley, S.,
Scholes, C.P., and Cunningham, R.P. (1995) Endonuclease III interactions
with DNA substrates: 1. Binding and footprinting studies with
oligonucleotides containing a reduced apyrimidinic site. Biochemistry
34,2528-2536. Click here
to see abstract.
Xing, D. Dorr, R.,
Cunningham, R.P., and Scholes, C.P. (1995) Endonuclease III interactions
with DNA substrates: 2. The DNA repair enzyme endonuclease III
binds differently to intact and to AP DNA substrates as shown
by tryptophan fluorescence quenching. Biochemistry 34,2537-2544.
Click here
to see abstract.
Tainer, J.A., Thayer,
M.M. and Cunningham, R.P. (1995) DNA repair proteins. Curr. Opin.
Struct. Biol. 5,20-26.
Mol, C.D., Kuo,
C-F., Thayer, MM, Cunningham, R.P. and Tainer, J.A. (1995) Structure
and function of the multifunctional DNA repair enzyme exonuclease
III. Nature 374,381-386. Click here
to see abstract.
Barzilay, G., Mol,
C.D. Robson, C.N. Walker, L.J. Cunningham, R.P., Tainer, J.A.,
and Hickson, I.D. (1995) Identification of critical active-site
residues in the multifunctional human DNA repair enzyme HAP1.
Nature Structural Biology 2,561-568. Click here
to see abstract.
Thayer, M.M., Ahern,
H., Xing, D., Cunningham, R.P., and Tainer, J.A. (1995) Novel
DNA binding motifs in the DNA-repair enzyme endonuclease III structure.
EMBO J. 14,4108-4120. Click here
to see abstract.
Zuo, S., Boorstein,
R.J., Cunningham, R.P. and Teebor, G.W. (1995) Comparison of the
effects of UV irradiation on 5-methylsubstituted and unsubstituted
pyrimidines in alternating Py-Pu sequences in DNA. Biochemistry
34,11582-11590.
Hilbert, T.P., Boorstein,
R.J., Xing, D., Kung, P.H., Bolton, P.H., Cunningham, R.P. and
Teebor, G.W. (1996) Purification of a mammalian homolog of E.
coli endonuclease III: Identification of a calf thymus pyrimidine
hydrate/thymine glycol/AP lyase by crosslinking the enzyme to
a thymine glycol-containing DNA-oligonucleotide. Biochemistry
35,2505-2511.
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