Next Gen Sequencing Project Proposal formPlease, use this form to tell us about the details of your Next Gen sequencing project
Researcher Name Principal Investigator Name Institution Researcher E-mail Address Researcher Phone Number Number of Samples Select Type of Sample Select Type of Sample - None -CellsTissueTotal RNAPurified DNAmRNAsmall RNAcDNAIllumina LibraryOther - Please Specify Enter other… Organism Organism - None -HumanMouseRatOther - Please Specify Enter other… Nucleic Acid Extraction/QC Do you require RNA Isolation? Do you require RNA Isolation? - None -Total RNATotal RNA + miRNANot applicableOther Specify RNA isolation desired Do you require DNA Isolation? Do you require DNA Isolation? - None -genomic DNANot applicableOther Specify DNA isolation desired RNA/DNA Quality Control request Nanodrop Agilent Bioanalyzer None, will be provided by customer Library Preparation/QC Is library preparation for Illumina NextSeq 2000 required? - None -Yes NoRequest Advice NGS on Illumina NextSeq 2000 Do you require NGS? - None -YesNo Do you require SE or PE sequencing? - None -Single End SequencingPaired End Sequencing What are your desired run parameters? - None -P1 - 1x50P1 - 2x150P1 - 2x300P2 - 2x50P2 - 2x100P2 - 2x150P2 - 2x300P3 - 1x50P3 - 2x50P3 - 2x100P3 - 2x150 How many total reads are required? - None -P1 - 100M SEP1 - 200M PEP2 - 400M SEP2 - 800M PEP3 - 1.2B SEP3 - 2.4B PE If you are unsure about your sequencing depth requirements, please contact the core. Link to Illumina for sequencing depth recommendations Link to Illumina for Available flow cells Do you require Data Analysis? - None -YesNo Comments Submit Leave this field blank
